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crispr cas9 assisted recombineering78  (Addgene inc)


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    Structured Review

    Addgene inc crispr cas9 assisted recombineering78
    Crispr Cas9 Assisted Recombineering78, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+cas9+assisted+recombineering78/pCas+(Plasmid+%2362225)/pm41365856-215-52-57
    Average 94 stars, based on 100 article reviews
    crispr cas9 assisted recombineering78 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy.
    Article Snippet: The coding sequence for mdh2 CT4-1 Cn45 and mdh Gs were synthesized by Eurofins. .. Themdh2CT4-1CnH165N and mdh2 CT4-1 Cn H165N F279I mutants were obtained by PCR amplification of the vector containing mdh2 CT4-1 Cn using a mutagenic forward primer with overhang and subsequent isothermal assembly. pET21a expression plasmid containing the activator act Bm MGA3 (Uniprot No. I3EA59) were received from SINTEF, Norway44,54. pCas encoding cas9 for CRISPR-Cas9-assisted recombineering78 was obtained from Addgene (plasmid #62225). pTargetF_sacB was used as the vector for the sgRNA and was generated by integrating the sacB gene into pTargetF78, obtained from Addgene (plasmid #62226). .. The sacB gene from pK18mob_sacBwas amplified by PCR, and the product as well as pTargetF digested with XhoI and BglII (Thermo Fisher Scientific, USA).

    Amplification:

    Article Title: Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy.
    Article Snippet: The coding sequence for mdh2 CT4-1 Cn45 and mdh Gs were synthesized by Eurofins. .. Themdh2CT4-1CnH165N and mdh2 CT4-1 Cn H165N F279I mutants were obtained by PCR amplification of the vector containing mdh2 CT4-1 Cn using a mutagenic forward primer with overhang and subsequent isothermal assembly. pET21a expression plasmid containing the activator act Bm MGA3 (Uniprot No. I3EA59) were received from SINTEF, Norway44,54. pCas encoding cas9 for CRISPR-Cas9-assisted recombineering78 was obtained from Addgene (plasmid #62225). pTargetF_sacB was used as the vector for the sgRNA and was generated by integrating the sacB gene into pTargetF78, obtained from Addgene (plasmid #62226). .. The sacB gene from pK18mob_sacBwas amplified by PCR, and the product as well as pTargetF digested with XhoI and BglII (Thermo Fisher Scientific, USA).

    Plasmid Preparation:

    Article Title: Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy.
    Article Snippet: The coding sequence for mdh2 CT4-1 Cn45 and mdh Gs were synthesized by Eurofins. .. Themdh2CT4-1CnH165N and mdh2 CT4-1 Cn H165N F279I mutants were obtained by PCR amplification of the vector containing mdh2 CT4-1 Cn using a mutagenic forward primer with overhang and subsequent isothermal assembly. pET21a expression plasmid containing the activator act Bm MGA3 (Uniprot No. I3EA59) were received from SINTEF, Norway44,54. pCas encoding cas9 for CRISPR-Cas9-assisted recombineering78 was obtained from Addgene (plasmid #62225). pTargetF_sacB was used as the vector for the sgRNA and was generated by integrating the sacB gene into pTargetF78, obtained from Addgene (plasmid #62226). .. The sacB gene from pK18mob_sacBwas amplified by PCR, and the product as well as pTargetF digested with XhoI and BglII (Thermo Fisher Scientific, USA).

    Expressing:

    Article Title: Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy.
    Article Snippet: The coding sequence for mdh2 CT4-1 Cn45 and mdh Gs were synthesized by Eurofins. .. Themdh2CT4-1CnH165N and mdh2 CT4-1 Cn H165N F279I mutants were obtained by PCR amplification of the vector containing mdh2 CT4-1 Cn using a mutagenic forward primer with overhang and subsequent isothermal assembly. pET21a expression plasmid containing the activator act Bm MGA3 (Uniprot No. I3EA59) were received from SINTEF, Norway44,54. pCas encoding cas9 for CRISPR-Cas9-assisted recombineering78 was obtained from Addgene (plasmid #62225). pTargetF_sacB was used as the vector for the sgRNA and was generated by integrating the sacB gene into pTargetF78, obtained from Addgene (plasmid #62226). .. The sacB gene from pK18mob_sacBwas amplified by PCR, and the product as well as pTargetF digested with XhoI and BglII (Thermo Fisher Scientific, USA).

    CRISPR:

    Article Title: Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy.
    Article Snippet: The coding sequence for mdh2 CT4-1 Cn45 and mdh Gs were synthesized by Eurofins. .. Themdh2CT4-1CnH165N and mdh2 CT4-1 Cn H165N F279I mutants were obtained by PCR amplification of the vector containing mdh2 CT4-1 Cn using a mutagenic forward primer with overhang and subsequent isothermal assembly. pET21a expression plasmid containing the activator act Bm MGA3 (Uniprot No. I3EA59) were received from SINTEF, Norway44,54. pCas encoding cas9 for CRISPR-Cas9-assisted recombineering78 was obtained from Addgene (plasmid #62225). pTargetF_sacB was used as the vector for the sgRNA and was generated by integrating the sacB gene into pTargetF78, obtained from Addgene (plasmid #62226). .. The sacB gene from pK18mob_sacBwas amplified by PCR, and the product as well as pTargetF digested with XhoI and BglII (Thermo Fisher Scientific, USA).

    Generated:

    Article Title: Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy.
    Article Snippet: The coding sequence for mdh2 CT4-1 Cn45 and mdh Gs were synthesized by Eurofins. .. Themdh2CT4-1CnH165N and mdh2 CT4-1 Cn H165N F279I mutants were obtained by PCR amplification of the vector containing mdh2 CT4-1 Cn using a mutagenic forward primer with overhang and subsequent isothermal assembly. pET21a expression plasmid containing the activator act Bm MGA3 (Uniprot No. I3EA59) were received from SINTEF, Norway44,54. pCas encoding cas9 for CRISPR-Cas9-assisted recombineering78 was obtained from Addgene (plasmid #62225). pTargetF_sacB was used as the vector for the sgRNA and was generated by integrating the sacB gene into pTargetF78, obtained from Addgene (plasmid #62226). .. The sacB gene from pK18mob_sacBwas amplified by PCR, and the product as well as pTargetF digested with XhoI and BglII (Thermo Fisher Scientific, USA).



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    crispr cas9 assisted recombineering78  (Addgene inc)
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    Addgene inc crispr cas9 assisted recombineering78
    Crispr Cas9 Assisted Recombineering78, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+cas9+assisted+recombineering78/pCas+(Plasmid+%2362225)/pm41365856-215-52-57
    Average 94 stars, based on 1 article reviews
    crispr cas9 assisted recombineering78 - by Bioz Stars, 2026-10
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    		  Buy from Supplier

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